Safety Evaluation of Nano Iron Zero Valente Green Synthesized: A Comparative Study
نویسندگان
چکیده مقاله:
Objective(s): Nowadays, examining the toxicity of nanoparticles including the synthesized and functionalized iron nanoparticles using methods like green synthesis is highly considered, due to their increasing usage in various fields of medicine, biology, industrial, and pollution removal. Hence, in this study, the toxicity of the zero valent iron nanoparticles synthesized by plant-Myrtus communis (MC-ZVINP) was investigated. Methods: Human normal Foreskin Fibroblast (HFF) cells were used for cytotoxicity examination using MTT method. Also, biochemical factors such as liver enzymes level, and factors such as the number of white and red globules, lymphocytes, platelets, amount of blood hemoglobin, and histopathological test of liver tissue in laboratory small rats were examined after intraperitoneal injections of the MC-ZVINP with different concentrations daily and a duration of 3-month, with the groups receiving trivalent iron, the extract of plant-case, and normal saline. Results: Cytotoxicity concentration of iron-case nanoparticles was obtained for 50% of HFF cells (CC50=149.23±4.45μg/mL). The results obtained from the blood factors examination showed a decreased the serum level of liver enzymes as well as an increase in the number of red and white globules and hemoglobin rate in mice receiving iron nanoparticles compared to the trivalent iron receiving group. Receiving the concentrations of 100 and 200 mg/kg/bw of iron nanoparticles have caused the incidence of mild and moderate inflammation in the liver of mice. Conclusions: Generally, it can be concluded that, the MC-ZVINP have shown no significant toxicity on the levels of blood cells, enzymes, and liver tissue.
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عنوان ژورنال
دوره 5 شماره 2
صفحات 160- 170
تاریخ انتشار 2020-04-01
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